| Studies on enzyme immunoassay for determining progesterone of bovine plasma and its clinical application I. Optimizing double antibody for progesterone in enzyme immunoassay |
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Chung-boo Kang, Jong-uk Shin, Sang-yong Choe |
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Department of Veterinary Medicine, College of Agriculture, Gyeongsang National University |
| Enzyme immunoassay(EIA)에 의한 소의 혈중 progesterone 측정과 이의 응용에 관한 연구 I. 이항체(二項體)의 최적조건에 관한 연구 |
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강정부, 신종욱, 최상용 |
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경상대학교 농과대학 수의학과 |
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| Abstract |
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This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay. The optimum conditions of assay-system, double (first and second) antibody and carrier (normal rabbit serum) were investigated. The optimum dilution rate of first antibody, second antibody and normal rabbit serum was $10{ imes}10^3$ to $15{ imes}10^3$, 20 and $1{ imes}10^3$ times, respectively. |
| Key Words:
bovine serum, ${eta}$-galactosidase, liquid phase, double antibody, enzyme immunoassay |
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