| Studies on enzyme immunoassay for determining progesterone of bovine plasma and its clinical application. II. Establishment of enzyme immunoassay for progesterone |
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Chung-boo Kang, Jong-uk Shin, Sang-yong Choe |
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College of Veterinary Medicine, Gyeongsang National University |
| Enzyme immunoassay(EIA)에 의한 소의 progesterone 측정과 이의 응용에 관한 연구 II. Progesterone 측정에 대한 효소면역측정방법(酵素免疫測定方法)의 확립 |
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강정부, 신종욱, 최상용 |
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경상대학교 수의과대학 |
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| Abstract |
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This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay. The optimum conditions of assay-system, enzyme conjugate and gelatin were invested. The sensitivity, recovery rate and reproducibility by this assay were also analyzed. The results obtained were as follows: 1. The suitable supplementation level of gelatin was 0.2%. As the gelatin level increased to 1%, coefficient variation of interassay was shown to be irregular. 2. The optimum dilution rate of enzyme conjugate was 30 times. Enzyme activity was greatly fluctuated depending on the minor condition of enzyme conjugate technique. Therefore, it was considered to be checked when determined. 3. The sensitivity of the assay was 12 pg/tube. 4. Recovery rate was decreased when the amount of sample was too little or too much, but the recovery rate was high as 97.8% when the amount of sample between 50 and $200{mu}l$. 5. Mean intra-assay and inter-assay coefficient of variation was 4.5% and 5.9%, respectively. By using liquid phase double antibody enzyme immunoassay, progesterone in plasma can be detected, and also this assay system is applicable to study on physiological function of progesterone and to diagnosis of reproductive disorders. |
| Key Words:
progesterone, enzyme conjugate, recovery rate, assay system, enzyme immunoassay |
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