| Comparison of sheep erythrocytes and Korean native goat erythrocytes-rosette forming rate of pig peripheral blood mononuclear cells |
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Young-jin Kim, Hee-jong Song, Jong-myeon Kim, Myeong-dai Kang, Chang-yong Yoon, Tae-joong Kim |
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College of Veterinary Medicine, Chonbuk National University |
| 돼지 말초혈액 단핵세포의 면양 및 재래산양 적혈구 rosette 형성능 비교 |
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김영진, 송희종, 김종면, 강명대, 윤창용, 김태중 |
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전북대학교 수의과대학 |
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| Abstract |
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To develope the methods for isolation and enumeration of lymphocyte subpopulations in pigs, we carried out the rosette-assay using sheep erythrocytes(SRBC) and Korean native goat erythrocytes(GRBC) as a target cells. To enumerate T lymphocytes, E-rosette methods were applied with RBC treated with various concentration of polymers such as Aet and Dex, singly or in combination. And to enumerate B lymphocytes, EAand EAC-rosette assay was adopted. The results were as follows; 1. E-RFR with polymer-untreated SRBC and GRBC was $32.9{pm}7.9%$ and $31.3{pm}9.4%$, respectively. On the other hand, RFR with 0.1M Aet plus 8% Dex treated SRBC and GRBC was increased about two-fold($67.8{pm}7.4%$ and $69.8{pm}8.5%$), respectively. 2. EA-RFR with SRBC and GRBC were $ 39.1{pm}10.2%$ and $32.6{pm}6.1%$, respectively. 3. EAC-RFR with SRBC and GRBC were $27.6{pm}7.0%$ arld $21.0{pm}3.2%$, respectively. These results showed that both SRBC and GRBC could be recommanded as a binding cells for rosetteassay to isolate of lymphocyte-subpopulations in pigs. |
| Key Words:
Rosette assay, sheep erythrocytes, Korean native goat erythrocytes, peripheral blood mononuclear cells, pig |
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