Korean Journal of Veterinary Research 1992;32(2):175-179.
Comparison of sheep erythrocytes and Korean native goat erythrocytes-rosette forming rate of pig peripheral blood mononuclear cells
Young-jin Kim, Hee-jong Song, Jong-myeon Kim, Myeong-dai Kang, Chang-yong Yoon, Tae-joong Kim
College of Veterinary Medicine, Chonbuk National University
돼지 말초혈액 단핵세포의 면양 및 재래산양 적혈구 rosette 형성능 비교
김영진, 송희종, 김종면, 강명대, 윤창용, 김태중
전북대학교 수의과대학
To develope the methods for isolation and enumeration of lymphocyte subpopulations in pigs, we carried out the rosette-assay using sheep erythrocytes(SRBC) and Korean native goat erythrocytes(GRBC) as a target cells. To enumerate T lymphocytes, E-rosette methods were applied with RBC treated with various concentration of polymers such as Aet and Dex, singly or in combination. And to enumerate B lymphocytes, EAand EAC-rosette assay was adopted. The results were as follows; 1. E-RFR with polymer-untreated SRBC and GRBC was $32.9{pm}7.9%$ and $31.3{pm}9.4%$, respectively. On the other hand, RFR with 0.1M Aet plus 8% Dex treated SRBC and GRBC was increased about two-fold($67.8{pm}7.4%$ and $69.8{pm}8.5%$), respectively. 2. EA-RFR with SRBC and GRBC were $ 39.1{pm}10.2%$ and $32.6{pm}6.1%$, respectively. 3. EAC-RFR with SRBC and GRBC were $27.6{pm}7.0%$ arld $21.0{pm}3.2%$, respectively. These results showed that both SRBC and GRBC could be recommanded as a binding cells for rosetteassay to isolate of lymphocyte-subpopulations in pigs.
Key Words: Rosette assay, sheep erythrocytes, Korean native goat erythrocytes, peripheral blood mononuclear cells, pig

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