Restriction endonuclease analysis of canine parvovirus DNA isolated in Korea

Park, Jong-hyeon; Song, Jae-young; Lee, Jung-bok; Hyun, Bang-hun; An, Soo-hwan; Jun, Moo-hyung

Korean Journal of Veterinary Research 1992;32(4):597-603.

Jong-hyeon Park¹, Jae-young Song¹, Jung-bok Lee¹, Bang-hun Hyun¹, Soo-hwan An¹, Moo-hyung Jun²

¹Veterinary Research institute
²College of Veterinary Medicine, Chungnam National University

국내에서 분리된 Canine parvovirus DNA의 제한효소 분석

박종현¹, 송재영¹, 이중복¹, 현방훈¹, 안수환¹, 전무형²

¹가축위생연구소
²충남대학교 수의과대학

Abstract

To elucidate the molecular genetical properties of the canine parvoviruses isolated from the diseased puppies in the regions of Kyunggi and Chungnam provinces, the replicative form (RF) DNA of four field isolates were compared with those of two attenuated vaccine strains and a reference strains of CPV by restriction endonuclease analysis (REA). REA by Hinf I showed that three CPV isolates except CPV-V15 had an identical banding pattern with two vaccine strains, one standard strain and feline panleukopenia virus (FPLV). In CPV-V15 strain the fourth fragment of DNA with 800 bp was deleted. REA by Bgl II and Pst I indicated that CPV-V15 and FPLV had a bigger second fragment than those of the other strains of CPV. Meanwhile REA by Bam HI revealed that all the field isolates and vaccine strains used in this experiment showed similar banding patterns.

Key Words: canine parvovirus, replicative DNA, restriction endonuclease analysis