Korean Journal of Veterinary Research 1992;32(4):597-603.
Restriction endonuclease analysis of canine parvovirus DNA isolated in Korea
Jong-hyeon Park1, Jae-young Song1, Jung-bok Lee1, Bang-hun Hyun1, Soo-hwan An1, Moo-hyung Jun2
1Veterinary Research institute
2College of Veterinary Medicine, Chungnam National University
국내에서 분리된 Canine parvovirus DNA의 제한효소 분석
박종현1, 송재영1, 이중복1, 현방훈1, 안수환1, 전무형2
1가축위생연구소
2충남대학교 수의과대학
Abstract
To elucidate the molecular genetical properties of the canine parvoviruses isolated from the diseased puppies in the regions of Kyunggi and Chungnam provinces, the replicative form (RF) DNA of four field isolates were compared with those of two attenuated vaccine strains and a reference strains of CPV by restriction endonuclease analysis (REA). REA by Hinf I showed that three CPV isolates except CPV-V15 had an identical banding pattern with two vaccine strains, one standard strain and feline panleukopenia virus (FPLV). In CPV-V15 strain the fourth fragment of DNA with 800 bp was deleted. REA by Bgl II and Pst I indicated that CPV-V15 and FPLV had a bigger second fragment than those of the other strains of CPV. Meanwhile REA by Bam HI revealed that all the field isolates and vaccine strains used in this experiment showed similar banding patterns.
Key Words: canine parvovirus, replicative DNA, restriction endonuclease analysis


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