Korean Journal of Veterinary Research 1993;33(2):269-275.
Studies on the rabbit viral hepatitis : Immunohistochemical observations
Cha-soo Lee1, Tae-kyun Shin2, Youn-ju Choi1, Kyu-sik Jeong1, Jong-sik Jyeong3
1College of Veterinary Medicine, Kyungpook National University
2Dep of Veterinary Medicine, Cheju National University
3Kyungpook Animed Health Experimental Institute
토끼의 바이러스성 간염에 관한 연구 : 면역조직화학적 관찰
이차수1, 신태균2, 최윤주1, 정규식1, 정종식3
1경북대학교 수의과대학
2제주대학교 수의학과
3경북가축위생시험소
Abstract
Tissue distribution of RHDV in rabbits were examined by immunofluorescence and ABC methods. Tissues including liver, spleen, kidneys, lungs and brain were frozen, cut in a crycut, and fixed in 10% buffered formalin, embedded in paraplast, and cut $5{sim}7{mu}m$ thickness. Sections were immunostained Tissue distribution of RHDV in rabbits were examined by immunofluorescence and ABC methods. Tissues including liver, spleen, kidneys, lungs and brain were frozen, cut in a crycut, and fixed in 10% buffered formalin, embedded in paraplast, and cut $5{sim}7{mu}m$ thickness. Sections were immunostained with primary antiserum and conjugated second antibodies as recommended by manufacturer. None of the cultures tested showed virus-induced phenomena. Immunoreactive products were commonly found in the liver, in some cases there were also positive staining in the spleen and kidneys. Other organs showed weak or insignificant immunoreactions. By ABC method on the formalin-fixed, paraffin-embedded liver tissues, strong immunoreactivity was found in the periportal triad lesions and peripheral lesions of the hepatic lobules. Immunoreactive products showed diffuse fine granular in the cytoplasm of hepatocytes and sinusoidal cells. In some cells, immunoproducts marginate at the periphery of the cells. The intensive staining of the cytoplasm of infected cells allowed their exact differentiation from surrounding uninfected cells. The positive area involved coincided with histopathological lesion on serial liver sections. In conclusion, liver was proved to be a consistent target organ in RHD, and the immunoperoxidase method in the section of formalin-fixed, paraffin-embedded hepatic tissue could be broadly used for the routine diagnosis of the disease.
Key Words: RHD, viral hepatitis, immunofluorescence, ABC immunohistochemistry


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