Immunohistochemical studies of glucagon and somatostatin in the pancreas of the Korean tree squirrel. Sciurus vlugar is corea |
Hyeung-sik Lee1, Jae-hyun Lee2 |
1Department of Biology, Kyungsan University 2College of Veterinary Medicine, Kyungpook National University |
청설모췌장의 glucageon과 somatostatin 세포의 면역조직학적 연구 |
이형식1, 이재현2 |
1경산대학교 생물학과 2경북대학교 수의과대학 |
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Abstract |
The pancreatic endocrine cells, glucagon and somatostation, of the Korean tree squirrel. Sciurus vulgais corea, were investigation by means of light and electron microscopic immunohistochemistry using the PAP and protein A-gold techniques. Glucagon-immunoreactive cells were distributed the periphery and occasinonaly central region of the pancreatic islets. Also, isolated cell was found between the pancreatic ancinar cells. The glucagon cells contraine granules with a diameter of 240~320nm and the electron dense core usually surrounded by a halo of less dense granular material. The core of granule was labelled strongly with gold particles. Somatostatin-immunoreactive cells were weakly stained in scattered along the peripheral pancreatic islets and were distributed as singly or small groups with in the pancreatic acinar cells. The somatostatin cells were spherical with a diameter of 250~275nm, moderately electron opaque (Gold particles were mostly demonstrated on the entire granule. |
Key Words:
Korean tree squirrel, glucagon, somatostatin, pancreas, protein A-gold technique |
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