Immunohistochemical study on some calcium binding proteins and neurotransmitters in suprachiasmatic nucleus of the Korean native goat

Song, Seung-hoon; Lee, Heunshik S.; Lee, In-Se

Korean Journal of Veterinary Research 2001;41(2):139-146.

Seung-hoon Song, Heunshik S. Lee, In-Se Lee

College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University

한국재래산양 시각교차위핵 내 몇 가지 칼슘결합단백질과 신경전달물질의 분포에 관한 면역조직화학적 연구

송승훈, 이흥식, 이인세

서울대학교 수의과대학 및 농생명공학부

Abstract

This study was carry out to identify the distribution of calcium binding proteins; calbindin(CB), calretinin(CR) and parvalbumin(PA) in the suprachiasmatic nucleus(SCN) of the Korean native goat by immunohistochemical methods. The expression of substance P(SP), calcitonin gene-related peptide(CG-RP), neuropeptide Y(NPY), vasoactive intestinal polypeptide(VIP) and galanin(GAL) were also investigated. CR-immunoreactivity was found in both of the cell bodies and fibers in the SCN, which the CB-immunoreactivity was observed only in the fibers. The immunoreactivity for VIP was observed in both the cell bodies and fibers, but SP-, NPY, GAL-immunoreactivities were only found in the fibers. CGRP-immunoreactivity was not seen in cell body and fibers. These results suggest that VIP, SP, NPY and GAL play a neuromodulatory or/ and neurotransmitter roles in cooperation with CB and CR in SCN of the Korean native goat.

Key Words: suprachiasmatic nucleus, calcium binding protein, neurotransmitter, immunohistochemistry, Korean native goat