| Development of analytical method for tylosin residues in animal products and its application |
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Tae-jong Kim, Jong-bae Kim, Chi-ho Lee, Won-chang Lee, Hwa-joong Yoon |
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College of Animal Husbandry and Animal Resources Research Center, Kon-Kuk University |
| 축산물중의 Tylosin 잔류물질 검사를 위한 분석법의 개발과 그 이용에 관한 연구 |
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김태종, 김종배, 이치호, 이원창, 윤화중 |
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건국대학교 축산대학 및 동물자원연구센타 |
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| Abstract |
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This study was performed to develop immnoassay method of detecting the residual tylosin and to investigate the residues using HPLC(high performance liquid chromatography) in animal products. Obtained results are the followings: 1. To develop immunoassay method, the conjugation of activated tylosin tartarate ester derivatives and BSA (bovine serum albumin) was certified at the 290nm of maximal absorbance which tylosin tartrate have. 2. The titration of anti-serum produced from rabbit immunized with the conjugator as an immunogen was too low to analyze the tylosin. 3. The residual tylosin can be detected by 0.2 ppm using HPLC. 4. Recovery of tylosin from spiked pork samples measured using HPLC was $87.4{pm}4.0%$. 5. When the levels of tylosin residues in swine liver and kindney were measured on HPLC. The level was over the maximum tolerance level in one out of ten samples of each organ. |
| Key Words:
immunoassay method, the residual tylosin, HPLC, the conjugator |
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