Development of an ELISA kit for the detection of residual sulfadimethoxine in edible animal products

Kim, Woo-taek; Kim, Seong-hee; Yoon, Byoung-su; Lim, Yoon-kyu

Korean Journal of Veterinary Research 2000;40(3):601-609.

Woo-taek Kim¹, Seong-hee Kim², Byoung-su Yoon³, Yoon-kyu Lim²

¹Cheju Institute for Livestock Promotion
²Department of Veterinary Medicine, Cheju National University
³Department of Biology, College of Natural Science Kyonggi University

축산물 잔류 sulfadimethoxine 검출용 ELISA kit 개발

김우택¹, 김성희², 윤병수³, 임윤규²

¹제주도 축산진흥원
²제주대학교 농과대학 수의학과
³경기대학교 이과대학 생물학과

Abstract

An enzyme linked immunosorbent assay (ELISA) was developed to screen residues of sulfadimethoxine (SDM) in edible animal products. An indirect competitive ELISA was allowed to compete with rabbit anti-SDM for binding to a limited amount of SDM-gelatin conjugate and SDM in serum samples. Sera was diluted 20 times with phosphate buffered saline (PBS) and boiled for 5 minutes to destruct immunoglobulins of serum. Detection limit of this competitive ELISA for SDM was 0.1 ppb or less. Among eight sulfonamide analogues tested for specifity, only sulfamonomethoxine showed significant cross-reaction in the assay. The EC-50 value for sulfamonomethoxine was 3.5 ppm. Recovery of SDM in spiked serum samples between 100 ppb and 500 ppb ranged from 110.7% to 128.9%.

Key Words: sulfadimethoxine, ELISA, swine serum