| Development of an ELISA kit for the detection of residual sulfadimethoxine in edible animal products |
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Woo-taek Kim1, Seong-hee Kim2, Byoung-su Yoon3, Yoon-kyu Lim2 |
1Cheju Institute for Livestock Promotion
2Department of Veterinary Medicine, Cheju National University
3Department of Biology, College of Natural Science Kyonggi University |
| 축산물 잔류 sulfadimethoxine 검출용 ELISA kit 개발 |
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김우택1, 김성희2, 윤병수3, 임윤규2 |
1제주도 축산진흥원
2제주대학교 농과대학 수의학과
3경기대학교 이과대학 생물학과 |
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| Abstract |
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An enzyme linked immunosorbent assay (ELISA) was developed to screen residues of sulfadimethoxine (SDM) in edible animal products. An indirect competitive ELISA was allowed to compete with rabbit anti-SDM for binding to a limited amount of SDM-gelatin conjugate and SDM in serum samples. Sera was diluted 20 times with phosphate buffered saline (PBS) and boiled for 5 minutes to destruct immunoglobulins of serum. Detection limit of this competitive ELISA for SDM was 0.1 ppb or less. Among eight sulfonamide analogues tested for specifity, only sulfamonomethoxine showed significant cross-reaction in the assay. The EC-50 value for sulfamonomethoxine was 3.5 ppm. Recovery of SDM in spiked serum samples between 100 ppb and 500 ppb ranged from 110.7% to 128.9%. |
| Key Words:
sulfadimethoxine, ELISA, swine serum |
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