| Distribution of growth hormone-releasing factor- and somatostatin-immunoreactive neurons in the hypothalamus of the Korean squirrel(sciurus vulgalis coreae) |
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Young-gil Jeong1, Hwa-young Son2, Won-kee Yoon2, Kil-soo Kim3, Moo-ho Won1, Si-yun Ryu2, Sung-whan Cho2, Moo-kang Kim2 |
1College of Medicine, Hallym University
2College of Veterinary Medicine, Chungnam National University
3Institute of Asan Life Science |
| 청서 시상하부의 growth hormone-releasing factor 및 somatostatin 면역반응신경세포의 분포 |
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정영길1, 손화영2, 윤원기2, 김길수3, 원무호1, 류시윤2, 조성환2, 김무강2 |
1한림대학교 의과대학 해부학교실
2충남대학교 수의과대학 형태학교실
3아산생명과학연구소 실험동물연구실 |
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| Abstract |
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This study was attempted to investigate the topographical distribution, shape and immunoreactivity of growth hormone-releasing factor(GRF)- and somatostatin(SOM)-immunoreactive neurons in the hypothalamus of the Korean squirrels(Sciurus vulgalis coreae). For the light microscopical examination of immunohistochemistry, the brains were fixed with 4% paraformaldehyde solution by means of intracardiac perfusion. And the frozen sections($40{mu}m$ thick) were stained immunohistochemically by ABC method. Distribution of GRF immunoreactive neurons($12-17{mu}m$) was highest in the paraventricular nucleus, moderate in the periventricular and supraoptic nuclei, and low in the arcuate nucleus and lateral hypothalamic area. Their immunoreactive fibers were found very high in the median eminence, moderately in the supraoptic, paraventricular and periventricular nuclei, and low in the arcuate nucleus and lateral hypothalamic area. SOM immunoreactive perikarya($14-18{mu}m$) were found moderately in the periventricular nucleus near the subependymal layer of the third ventricle, and low in the arcuate and suprachiasmatic nuclei. SOM immunoreactive fibers were found high in the median eminence, and moderately or low in the arcuate and periventricular nuclei. |
| Key Words:
growth hormone-releasing factor, somatostatin, hypothalamus, immunohistochemistry, Korean squirrel |
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