Korean Journal of Veterinary Research 1996;36(2):349-358.
Immunohistochemical detection of antigens in tissue sections of experimentally infected hamsters
Bok-gyeong Ku1, Soon-bok Kim2, Jung-hang Sur2, Oun-kyong Moon1, Suk-chan Jung1, Jung-moon Park3, Yong-ho Park4, Jin-san Mun1
1Veterinary Research Institute, Anyang RDA
2College of Veterinary Medicine Gyeongsang National University
3Korea Microbiology Lab. LTD,
4College of Veterinary Medicine Seoul National University
Leptospira canicola 실험적 감염햄스타의 조직절편내 면역조직화학적 균체항원검출
구복경1, 김순복2, 서정향2, 문윤경1, 정석찬1, 박정문3, 박용호4, 문진산1
1수의과학연구소
2경상대학교 수의과대학
3한국미생물연구소
4서울대학교 수의과대학
Abstract
The present study was done to detect Leptospira canicola antigens in paraffin sections from experimentally infected hamsters with PAP method. The anti-Leptospira canicola serum used as first antibody was prepared by immunizing rabbits with Leptospira canicola. Positive reactions were detected in interstitium, tubular epithelial cells and glomerulus of the kidney, and in the hepatic sinusoid. With PAP method, leptospiral antigens were detected at the 48 hours after bacterial infection, but isolation, silver stain, and dark field microscopic examination of the samples did 48 hours later than PAP method. The results suggested that PAP method is considered as a reliable tool for confirmative diagnosis of this disease.
Key Words: Leptospira canicola, PAP method


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