Isolation of adenovirus from porcine alveolar macrophages

Kweon, Chang-hee; Cho, Jae-jin; Kwon, Byung-joon; Song, Jae-young; Chae, Chanhee

Korean Journal of Veterinary Research 1997;37(1):177-183.

Chang-hee Kweon¹, Jae-jin Cho¹, Byung-joon Kwon¹, Jae-young Song¹, Chanhee Chae²

¹National Veterinary Research Institute
²College of Veterinary Medicine Seoul National University

돼지 폐포탐식세포로부터 adenovirus의 국내분리주 작성

권창희¹, 조재진¹, 권병준¹, 송재영¹, 채찬희²

¹수의과학연구소
²서울대학교 수의과대학

Abstract

One cytopathogenic virus was isolated in alveolar macrophages from pig without any apparent respiratory clinical signs. Biophysical properties and electron microscopy of the isolate showed the characteristics of adenovirus. Intracytoplasmic inclusion bodies were seen in virus-inoculated cells. The genetic analysis indicated the presence of DNA with the size of >20Kb. In a serological survey of 40 serum samples collected from two different farms in slaughter house, 9 sera were positive for neutralizing antibody against the isolate. The potential implications of the isolate as the causative agent in respiratory disorder were discussed.

Key Words: adenovirus, porcine alveolar macrophage, biophysical characteristics, serological survey in Korea