Studies on the use of computer aided semen analysis(CASA) technology for fertility prediction in Korean native cattle

Lee, Kang-nam; Lee, Byeong-chun; Hwang, Woo-suk

Korean Journal of Veterinary Research 1998;38(4):882-897.

Kang-nam Lee¹, Byeong-chun Lee², Woo-suk Hwang²

¹A-san animal hospital
²College of Veterinary Medicine, Seoul National University

한우에서 Computer aided semen analysis(CASA) 기법을 이용한 수태율 예측에 관한 연구

이강남¹, 이병천², 황우석²

¹아산동물병원
²서울대학교 수의과대학

Abstract

This experiment was conducted to predict the effects of motional characteristics on the fertility of Korean native cattle(KNC) by using CASA technology and in vitro fertilization system. Twenty-six KNC frozen semen straws were obtained from Korean KNC improvement department, livestock improvement main division, national livestock cooperatives federation in Korea. Specimens were allowed to thaw at $37^{circ}C$ for 30 sec in water bath. Semen analysis was performed on semen image analysis system(SIAS, Medical supply, Korea) adjusted to the gate settings and used the semen droplet ($5{mu}l$) placed on Makler counting chamber(Sefi medical instrument, Israel) prewarmed at $37^{circ}C$. The same person used the same micropipette to fill the Makler counting chamber. A total of 150 or more of sperms were analysed in each specimen by a single trained person by scanning at least 5 to 10 fields. The oocytes collection, in vitro maturation, IVF, in vitro culture and determination of the cleavage rate were performed by the technique, as described by Hwang et al (1997). Statistical analysis was done by linear regression with use of the Sigma plot program on a IBM personal computer. The cleavage rate in vitro fertilized oocyte was significantly correlated(P<0.05) with MOT, VCL, VSL, VAP, ALH, BCF and MAD, but not CON, LIN, STR, WOB, DNM, DNC and HYP in regressional analysis. The results show that some kinematic characteristics of frozen-thawed semen by CASA can be predict the fertility in in vitro model system.

Key Words: Korean native cattle, semen, CASA, motional kinematics, IVF, cleavage rate