| Morphometrical analysis of chicken Cryptosporidium on electron microscopy |
|
Nam-yong Park, Young-seop Kim, Chi-young Chung, Kyoung-oh Cho, Young-seok Park, Bong-joo Lee, Hyung-seon Park |
|
College of Veterinary Medicine, Chonnam National University |
| 닭 크립토스포리디움의 형태계측을 위한 전자현미경적 연구 |
|
박남용, 김영섭, 정치영, 조경오, 박영석, 이봉주, 박형선 |
|
전남대학교 수의과대학 |
|
|
| Abstract |
|
Morphometrical analysis of chicken Cryptosporidium baileyi in various stages of life cycle in the bursa of Fabricius were carried out by electron microscope to establish a differential point for identification of C baileyi. By avidin-biotin complex method, protozoans of the bursa of Fabricius were identified Cryptosporidium spp. The size and area on each developmental stages of C baileyi, as measured by Morphomat 10 attached to electron microscope were as follows. Trophozoites' size with range of $3.21{pm}0.70{ imes}2.49{pm}0.59{mu}m$, area with range of $118.82{pm}41.92{mu}m^2$; meronts' size $3.99{pm}1.07{ imes}2.96{pm}0.52{mu}m$, area $210.11{pm}57.11{mu}m^2$; merozoites' size $1.98{pm}0.43{ imes}0.60{pm}0.18{mu}m$, area $24.10{pm}5.97{mu}m^2$; microgametes' size $1.36{pm}0.83{ imes}0.50{pm}0.23{mu}m$, area $20.23{pm}6.73{mu}m^2$; macrogametes' size $4.57{pm}0.65{ imes}4.02{pm}0.55{mu}m$, area $258.37{pm}51.83{mu}m^2$; oocytes' size $4.39{pm}0.56{ imes}3.44{pm}0.50{mu}m$, area $187.21{pm}62.68{mu}m^2$. In conclusion, the size and area on each developmental stages of Cryptosporidium baileyi is different from that of other Gryptosporidia spp. It suggests, with considering tissue tropism and life cycle, morphometrical analysis can be quite a good way to identify C baileyi. |
| Key Words:
cryptosporidium baileyi, chicken, electron microscopy, morphometrical analysis |
|
PDF Links
Full text via DOI
Download Citation
