Diagnosis of canine distemper by in situ hybridization

Cho, Hyeon; Park, Nam-yong; Kim, Yong-hwan; Cho, Kyoung-oh; Park, Hyung-seon; Park, Young-seok; Lee, Bong-joo; Chung, Chi-young; Im, Hyung-ho

Korean Journal of Veterinary Research 1999;39(3):583-592.

Hyeon Cho, Nam-yong Park, Yong-hwan Kim, Kyoung-oh Cho, Hyung-seon Park, Young-seok Park, Bong-joo Lee, Chi-young Chung, Hyung-ho Im

College of Veterinary Medicine, Chonnam National University

In situ hybridization에 의한 개 디스템퍼의 진단

조현, 박남용, 김용환, 조경오, 박형선, 박영석, 이봉주, 정치영, 임형호

전남대학교 수의과대학

Abstract

We have developed the in situ hybridization(ISH) technique for rapid diagnosis of canine distemper(CD) which is the major infectious disease in dogs. In our experiment, we rapidly detected distribution of the specific canine distemper viral genome without disrupting morphology of tissues or cells. Two oligonucleotide probes for ISH were synthesized chemically and labelled 5' end with nonisotopic biotin by DNA synthesizer. The whole procedures of ISH was completed within 1~2 hours using the Microcapillary action system. On histological study, typical cytoplasmic or intranuclear inclusion bodies were observed in the trachea, bronchiole, brain, and urinary bladder with the presence of prominent red positive signals on ISH, indicating specific CDV genome from the paraffin-embedded tissues of infected 13 cases. The results showed ISH can be used as a rapid and effective diagnostic method for diagnosis of CD.

Key Words: canine distemper, in situ hybridization, CD, ISH