Rapid and Easy Detection of Porcine Epidemic Diarrhea Virus (PEDV) by in situ Hybridization

Park, Nam-yong; Cho, Ho-seong; Kim, Tae-ju; Park, Young-seok

Korean Journal of Veterinary Research 2003;43(3):477-483.

Nam-yong Park¹, Ho-seong Cho¹, Tae-ju Kim¹, Young-seok Park²

¹College of Veterinary Medicine, Chonnam National University
²Department of Companion Laboratory Animal Science, Kongju University

In Situ Hybridization에 의한 돼지 유행성설사증 (Porcine Epidemic Diarrhea)의 진단

박남용¹, 조호성¹, 김태주¹, 박영석²

¹전남대학교 수의과대학
²공주대학교 특수동물학과

Abstract

Molecular diagnostic techniques have been used to identify porcine epidemic diarrhea virus (PEDV), a causative agent of acute enteritis in swine, but they were difficult to be petformed and time-consuming. To detect PEDV in a rapid and easy way, we developed biotinylated cDNA probe for N gene encoding the nucleoproteins of PEDV. Formalin-fixed and paraffin-embedded tissues from 24 naturally infected pigs were used for the experiment. The ISH produced a positive reaction in all cases. When intestinal tissues were hybridized with PEDV probe, strong signals were seen in the villus enterocytes of the jejunum and ileum. Hybridization signals were also found in the duodenum from one pig and in colon from dnother. In conclusion, ISH with a biotinylated cDNA probe was provided to be a useful diagnostic method for detecting PEDV effectively in routinely processed tissue sections.

Key Words: porcine epidemic diarrhea virus, in situ hybridization, cDNA probe, PED