Korean Journal of Veterinary Research 2000;40(2):301-310.
Characteristics of Escherichia coli isolated from piglets with diarrhea ; antimicrobial susceptibility, genotypes of enterotoxins and pili and plasmid profiles
Joo-youn Park, Na-ri Shin, Yong-ho Park, Han-sang Yoo
College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
설사 자돈으로부터 분리한 Escherichia coli의 특성에 관한 연구 ; 항균제 감수성, 장독소 및 섬모의 유전형의 분포 및 plasmid profiles
박주연, 신나리, 박용호, 유한상
서울대학교 수의과대학 및 농생명공학부
Abstract
The antimicrobial susceptibility, genotypes of enterotoxins(LT, STa) and pili(K88, 987P), and plasmid profiles were investigated with 102 Escherichia coli isolated from piglets showing diarrhea in Korea. Almost of them were susceptible to ceftiofur(99%), cefquinone(97.1%). However they showed resistance to bacitracin(100%), streptomycin(98%), vancomycin(97%), trimethoprim/sulfamethoxazole(87.2%), tetracycline(84.3%) in antimicrobial susceptibility test. Moreover, all of the isolates demonstrated resistance to more than 2, and 78% of them were resistant to more than 8 of total 17 drugs. Multiplex PCRs for genotyping of enterotoxins(LT, STa) and pili(K88, 987P) were established with primers designed based on sequences from Genebank. Seventeen strains(16.6%) of the isolates had STa gene, 11 strains(10.8%) of them had both STa and LT genes, and 18 strains(17.8%) had K88 gene. But none of the isolates harbored a gene exclusively encoding LT. The gene encoding 987P pili was not found in all isolates. Fifty-four strains of 102 isolates(52.9%) had plasmid with various sizes ranging from 125kb to 1.1kb. Numbers of plasmid per isolates were also various, from 1 to 9. Distinctive relationship between plasmid profiles and genotypes of enterotoxins and pili in the isolates was not found. These results might provide the basic knowledge to establish strategies for the treatment and prevention of colibacillosis in piglets.
Key Words: Escherichia coli, antimicrobial susceptibility, genotypes of enterotoxins and pili, plasmid profiles


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