Isolation and prevalence of Setaria microfilariae in cattle

Moon, Moo-hong; Kang, Seung-won

Korean Journal of Veterinary Research 2000;40(2):339-343.

Moo-hong Moon¹, Seung-won Kang²

¹College of Veterinary Medicine, Kyungpook National University
²National Veterinary Research and Quarantine Service

소에서 Setaria속 사상충 자충의 분리와 감염상황

문무홍¹, 강승원²

¹경북대학교 수의과대학
²국립수의과학검역원

Abstract

Isolation and identification, prevalence and density of Setaria microfilariae(mf) were investigated in the blood of 406 cattle slaughtered at the abatoirs in Yungnam province from February 1999 to March 2000. Nine ml of blood from the jugular vein was collected in a test tube including EDTA respectively. The blood was mixed(10 : 1) with 0.2% Saponin in saline solution. This mixture was diluted again(1 : 10) with saline solution and stirred. This diluted mixture was filtered using Millipore membrane filter with a pore size of $3.0{mu}m$. Mf were recovered from the membrane in saline solution. Mf isolated were identified as Setaria digitata on the basis of morphologic characteristics. Infection rate of the mf of total 406 cattle was revealed as 6.40%. Seasonal prevalences of the mf were presented 9.46%(7/74) in Autumn, 7.05%(11/156) in Summer, 4.65%(4/86) in Winter and 4.44%(4/90) in Spring respectively. Average density of the mf of every 9ml blood in 26 positive cattle was revealed as 170(2-641) worms. Seasonal densities of the mf of 9ml blood were presented average 361(160-641) worms in Autumn, 205(16-620) worms in Summer, 108(6-204) worms in Spring, 6(2-11) worms in Winter respectively.

Key Words: setaria digitata, microfilaria, prevalence