Korean Journal of Veterinary Research 2000;40(3):533-541.
Studies on the safety of Brucella abortus RB51 vaccine I. Comparison of the biochemical and genetic characteristics of Brucella abortus RB51 vaccine strains
Jong-man Kim1, Sung-ryong Woo1, Ji-youn Lee1, Suk-chan Jung1, Seung-won Kang1, Jong-yeom Kim1, Yong-dhuk Yoon1, Sang-nae Cho2, Han-sang Yoo3, Steven C. Olsen4
1National Veterinary Research and Quarantine Service
2College of Medicine, Yonsei University
3College of Veterinary Medicine, Seoul National University
4National Animal Disease Center, United States Department Agriculture, United States of America
부루세라백신(RB51)의 안전성에 관한 연구 I. Brucella abortus RB51 백신균주의 생화학적 및 유전학적 성상비교
김종만1, 우승룡1, 이지연1, 정석찬1, 강승원1, 김종염1, 윤용덕1, 조상래2, 유한상3, 4
1국립수의과학검역원
2연세대학교 의과대학
3서울대학교 수의과대학
4미국 국립동물질병연구센터
Abstract
Biochemical and genetic analysis were carried out to investigate the potential recovery of pathogenecity or related mutations of Brucella abortus RB51 vaccine strains. RB51 strains were recovered from commercial vaccines, including related seed stocks from private companies in Republic of Korea, strain from USA, a reference strain from C university and a field isolate (Daehungjin) from aborted dairy cow after RB51 vaccination were compared with two identified virulent wild strains (S2308 and a field strain isolated from dairy cow in Korea) at the same conditions. All the strains examined, except identified pathogenic strains, revealed the identical characteristics to the original RB51 in biochemical properties, antigen and bacteriophage typing. Outer membrane protein (OMP) profiles from strains of RB51 showed the same patterns with standard RB51 in SDS-PAGE. In addition, Western blotting with the brucella specific monoclonal antibody also indicated that all the vaccine strains were completely deficient in their LPS compared to the pathogenic Br abortus strains. The differences in DNA structures among strains were also possible to detect after PCR. All vaccine strains, except S19, S1119-3, S1075, S544 and Br suis, were amplified a 178bp DNA fragment of eri-gene, and 364bp of IS711 elements. In contrast, 498bp DNA product was only found with Br abortus. Overall evidences in the present study confirmed that the RB51 strains for vaccine production in Korea did not originated from the phenomena of possible recovery of pathogenicity or related to any potential mutation event at all.
Key Words: Brucella abortus, RB51 vaccine, biochemical/genetic properties


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