Sequence analysis of the hypervariable region in VP2 gene of infectious bursal disease vaccine strains

Park, Yoo-jin; Kim, Soo-joung; Kwon, Hyuk-moo

Korean Journal of Veterinary Research 2001;41(3):333-342.

Yoo-jin Park¹, Soo-joung Kim², Hyuk-moo Kwon²

¹Kangwon provincial chunchon hunting grounds
²Department of Veterinary Medicine, Kangwon National University

Infectious bursal disease 백신주의 VP2 gene의 hypervariable region 분석

박유진¹, 김수정², 권혁무²

¹강원도립춘천수렵장
²강원대학교 수의학과

Abstract

To detect the genetic variations among infectious bursal disease (IBD) vaccine strains, the hypervariable region of VP2 gene of seven IBDV vaccine strains were amplified using reverse transcriptase/polymerase chain reation(RT/PCR). Ampllified PCR products of IBDV were cloned, sequenced, and compared with published sequences for IBDV. Vaccine strains (JOONG, HAN, B7, IB, BU2, G2, CIL) used in Korea and Korean field isolates (SH/92, K1, 310) had 81%(310 and HAN) ~ 98%(SH/92 and CIL) amino acid sequence similarity. Vaccine strains had 80%(HAN and IB) ~ 99%(JOONG and BU2) amino acid sequence similartiy. Intermediate plus vaccine strain, CIL was not substituted at positions 279(D $ ightarrow$ N) and 284(A $ ightarrow$ T), and conserved in serine-rich heptapeptide. At the two hydrophilic region, JOONG, IB and Bu2 strains had identical amino acid sequence comparing with STC strain. By phylogenetic analysis, JOONG and DAE strains were categorized in same group with BU2. The CIL and STC strains closely related but seperated from G2, HAN, B7 and IB strains.

Key Words: Infectious bursal disease virus(IBDV), hypervariable region,, VP2 gene, RT/PCR, sequence