Studies on genetic monitoring of inbred mice in conventional breeding unit

Lee, Heungshik S.; Kim, Chul-kyu

Korean Journal of Veterinary Research 2001;41(3):401-406.

¹College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
²National Institute of Toxicological Safety Research

일반사육시설 마우스의 유전적 오염에 대한 실태조사 연구

이흥식¹, 김철규²

¹서울대학교 수의과대학 및 농생명공학부
²국립독성연구소 일반독성부

Abstract

These studies were carried out to survey the genetic contamination of six inbred mice (A, BALB/c, C3H, C57BL/6, CBA and KK) produced and supplied from the conventional breeding unit for improving the quality of mice as experimental animal. We examined alleles of five loci (Akp-1, Car-2, Hbb, Es-1 and Trf) by the use of biochemical markers with celluose acetate electrophoresis. As the results of test, BALB/c, A, C3H, C57BL/6, CBA and KK showed standard alleles in Akp-1, Car-2 and Hbb. But Es-1 of A and C57BL/6 and Trf of A, C3H, C57BL/6 and CBA did allelic divergence in loci. These results suggest that the colonies of A, C3H, C57BL/6 and CBA were genetically contaminated. Therefore, we recommend to eliminate the genetically contaminated mice thoroughly, to check on genetic monitoring regularly and to consider a counterpaln for improving the quality control as soon as possible.

Key Words: genetic monitoring, genetic contamination, alleles, locus, biochemical marker