Comparison of serological methods for detection of avian influenza virus antibodies

Han, Myung-guk; Park, Kyoung-yoon; Kwon, Yong-kuk; Kim, Jae-hong

Korean Journal of Veterinary Research 2002;42(1):73-80.

Myung-guk Han¹, Kyoung-yoon Park², Yong-kuk Kwon¹, Jae-hong Kim¹

¹Avian Disease Division, National Veterinary Research and Quarantine Service
²Animal Health Division, Bayer Korea Ltd.

가금인플루엔자 바이러스 항체검출을 위한 혈청학적 진단법 비교

한명국¹, 박경윤², 권용국¹, 김재홍¹

¹국립수의과학검역원 조류질병과
²바이엘코리아(주) 임상병리실

Abstract

An enzyme-linked immunosorbent assay (ELISA) using purified hemagglutinin of swine influenza virus (H1N1) as antigen was developed for detection of antibody to avian influenza virus (AIV). The sensitivity and specificity of a developed and commercial available ELISA kits were compared with those of agar gel precipitation (AGP) test and hemagglutination inhibition (HI) test using sera collected from chickens under condition of field exposure. The concentration of antigen, serum dilution and concentration of enzyme-conjugated secondary antibody in developed ELISA (S-ELISA) were 0.5ug/100ul, 1:200 and 0.03ug/100ul, respectively. The correlation coefficients between S-ELISA and commercial ELISA and HI titers were 0.419 and 0.533, respectively. A significant correlation (p < 0.01) was not found between HI and ELISA titers. The S-ELISA was found to be as more sensitive and specific than the AGP test, showing 86.8% sensitivity and 85.3% specificity. It is suggested that the ELISA using the SIV as antigen may be useful method as an investigating tool for AIV serological surveillance.

Key Words: avian influenza, serology, ELISA