| The Safety of Brucella abortus strain RB51 in pregnant cattle |
|
Jong-man Kim1, Sung-ryong Woo1, Ji-youn Lee1, Suk-chan Jung1, Young-hwa Jean1, Jae-hoon Kim1, Chang-hee Kweon1, Young-dhuk Yoon1, Han-sang Yoo2 |
1National Veterinary Research and Quarantine Service
2College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University |
| 부루세라 RB51 백신의 임신소에 대한 안전성 |
|
김종만1, 우승룡1, 이지연1, 정석찬1, 진영화1, 김재훈1, 권창희1, 윤용덕1, 유한상2 |
1국립수의과학검역원
2서울대학교 수의과대학 |
|
|
| Abstract |
|
The safety of Brucella abortus strain RB51(SRB51) was investigated in dairy cows and Korean native cattle of 4~7th month of gestation. From experimentally inoculated cattle, 18 of 25 (72.0%) dairy cows, and 3 of 10 (30.0%) Korean native cattle were aborted or delivered premature fetus. There were no significant differences in the incidence of abortion depending on the inoculation route (intramuscular and subcutaneous) and dosages ($1{ imes}10^9$, $2.8{ imes}10^9$, and $4.0{ imes}10^9$ CFU). The antibodies to the SRB51 were measured by a dot blot enzyme-linked immunosorbent assay. The highest titers to SRB51 were detected between 5~7 weeks after inoculation and the specitic antibody could be detected up to 28 weeks after inoculation. The SRB51 was isolated from amnio-allantoic fluid, bronchial lymph node, mammary gland, and supramammary lymph node in 5 of 25 dairy cows during 4 weeks after either abortion or delivery. Although SRB51 was isolated from 4 of 24 aborted fetus or normally delivered calves at parturition time, it was not isolated during 4 weeks afterward. Eleven of twentyfive dairy cows showed the endometritis and/or necrosis until 6 weeks after delivery, no lesions were seen at 8 weeks after delivery and uterus from control dairy cows. The results of present study revealed that SRB51 might induce the clinical signs of brucellosis in the pregnant cattle at 4~7th month of gestation. |
| Key Words:
Brucella abortus, SRB51, safety, pregnant cattle, Dot-ELISA |
|
PDF Links
Full text via DOI
Download Citation
