| Production and diagnostic applications of monoclonal antibodies against porcine circovirus |
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Kyung-Mi Kim1, Ji-Hye Jeong1, Hong-Ki Min1, Seung-Chul Lee1, In-Soon Roh2, Shien-Young Kang1 |
1Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University
2National Veterinary Research and Quarantine Service |
| 돼지 써코바이러스에 대한 단크론항체 생산 및 진단적 응용 |
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김경미1, 정지혜1, 민홍기1, 이승철1, 노인순2, 강신영1 |
1충북대학교 수의과대학/동물의학연구소
2국립수의과학검역원 |
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| Abstract |
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Porcine circovirus type 2 (PCV-2) has been associated with various disease in pigs worldwide including postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). In this study, monoclonal antibodies (MAbs) against PCV were produced, characterized and applications of MAbs as diagnostic reagents were described. Spleen or lymph node cells from BALB/c mouse immunized respectively with PCV-1, PCV-2 or expressed PCV-2/ORF2 proteins in baculovirus were fused with SP2/0 myeloma cells using polyethylene glycol (PEG) and hybridoma cells producing PCV-1 or PCV-2-specific antibody were screened by an indirect immunofluorescence (IIF) test. A total of fifteen MAbs were produced against PCV. Six MAbs were PCV-1-specific and nine were PCV-2-specific. All PCV-1-specific MAbs reacted with only PCV-1 and all PCV-2-specific MAbs were reactive with only PCV-2 by IIF test. None of the MAbs was reactive with porcine reproductive and respiratory syndrome virus (PRRSV), porcine parvovirus (PPV), porcine rotavirus (PRV), and transmissible gastroenteritis virus (TGEV). Some PCV-2-specific MAbs recognized the PCV-2 infected porcine tissues by IIF or immunohistochemistry (IHC) assay. From this experiment, it was confirmed that MAbs produced in this study were PCV-specific and could be used as reliable diagnostic reagents for PCV-1/PCV-2 detection and differentiation. |
| Key Words:
porcine circovirus, monoclonal antibody, IIF, IHC |
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