Polymorphisms of the poly(C)-tract of porcine encephalomyocarditis virus (EMCV) isolated in Korea

Hyun, Bang-Hun; Kim, Hyo-Jin; Kim, In-Joong; Pyo, Hyun-Mi; Kim, Sun-Mi; Kim, Seong-Hee; Kim, Jae-Jo; Lim, Seong-In; Song, Jae-Young

Korean Journal of Veterinary Research 2010;50(3):221-229.

Bang-Hun Hyun, Hyo-Jin Kim, In-Joong Kim, Hyun-Mi Pyo, Sun-Mi Kim, Seong-Hee Kim, Jae-Jo Kim, Seong-In Lim, Jae-Young Song

Virology Department, National Veterinary Research and Quarantine Service

국내분리 돼지 뇌심근염바이러스의 poly(C)-tract의 다형태성

현방훈, 김효진, 김인중, 표현미, 김선미, 김성희, 김재조, 임성인, 송재영

국립수의과학검역원 바이러스과

Abstract

Encephalomyocarditis virus (EMCV) belongs to the genus Cardiovirus within the family Picornaviridae. EMCV has been recognized either as a cause of mortality in young pigs, due to acute myocarditis, or of reproductive failure in sows. An EMCV K3 strain was isolated from the heart and brain in a mummified and aborted swine fetus in 1989. For the molecular characterization of the poly(C)-tract of EMCV Korean isolates, K3 strain, viral RNA was extracted and digested with RNase T1, and analyzed the length of the poly(C)-tract by polyacrylamide gel electrophoresis. The poly(C) regions also were amplified by RT-PCR and sequenced. The present study shows that K3 strain of EMCV had a short polymorphic poly(C) tracts (5 to 30 C's) with sequences consisting of $C_9$, $C_{10}$, $C_{13}$, $C_{14}$, $C_{16}$, $C_{20}$, $CUC_{11}$, $C_8UCUC_3UC_{10}$, $C_9UCUC_3UC_{10}$, $C_{10}UCUC_3UC_{10}$, etc. These polymorphism of poly(C)-tracts of EMCV K3 strain implies the historical information of in vivo and/or in vitro passage.

Key Words: encephalomyocarditis virus, Korean isolate, poly(C) tract, polymorphism