| Molecular epidemiologic analysis of pathogenic Escherichia coli isolated from poultry in Korea |
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Myung-Suk Sung, Jin-Hyun Kim, Ki-Seuk Kim |
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College of Veterinary Medicine, Kyungpook National University |
| 국내 가금 유래 병원성 대장균의 분자역학적 분석 |
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성명숙, 김진현, 김기석 |
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경북대학교 수의과대학 |
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| Abstract |
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Among 203 avian pathogenic Escherichia coli (APEC) isolated from poultry with colibacillosis in korea, 14 isolates were selected from total 68 isolates transferred R plasmid and classified into 5 groups on the basis of antimicrobial minimal inhibitory concentration (MIC) pattern, farm source and O serotype. An association between clonal origin and R plasmid of them was investigated by R plasmid profile, restriction endonuclease analysis and pulsed-field gel electrophoresis (PFGE). The strains that showed the same or very similar antimicrobial MIC pattern, but different farm source and O serotype, revealed different PFGE pattern, which seemed to be different clonal origin. And the strains that showed the same MIC pattern and O serotype, revealed different PFGE pattern, seemed to be originated from different clone. Also the strains showing the same MIC pattern and farm source, but different O serotype, revealed to be different clonal origin. The strains that showed the same or similar MIC pattern, farm source, and O serotype, revealed identical or similar PFGE pattern, which seemed to belong to be one clone. Meanwhile, horizontal transfer of R plasmid seems to be common in APEC with regardless of O serotype and clone of the strains. These results indicate that rapid and accurate epidemiological survey of APEC can be possible by the combination of O serotyping, plasmid profiling and PFGE analysis following the classification of them into groups of antimicrobial drug resistance pattern. |
| Key Words:
avian pathogenic Escherichia coli (APEC), molecular epidemiological analysis, O serotype, pulsed-field gel electrophoresis (PFGE), restriction endonuclease analysis (REA) |
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