Korean Journal of Veterinary Research 2010;50(3):239-246.
Molecular epidemiologic analysis of pathogenic Escherichia coli isolated from poultry in Korea
Myung-Suk Sung, Jin-Hyun Kim, Ki-Seuk Kim
College of Veterinary Medicine, Kyungpook National University
국내 가금 유래 병원성 대장균의 분자역학적 분석
성명숙, 김진현, 김기석
경북대학교 수의과대학
Abstract
Among 203 avian pathogenic Escherichia coli (APEC) isolated from poultry with colibacillosis in korea, 14 isolates were selected from total 68 isolates transferred R plasmid and classified into 5 groups on the basis of antimicrobial minimal inhibitory concentration (MIC) pattern, farm source and O serotype. An association between clonal origin and R plasmid of them was investigated by R plasmid profile, restriction endonuclease analysis and pulsed-field gel electrophoresis (PFGE). The strains that showed the same or very similar antimicrobial MIC pattern, but different farm source and O serotype, revealed different PFGE pattern, which seemed to be different clonal origin. And the strains that showed the same MIC pattern and O serotype, revealed different PFGE pattern, seemed to be originated from different clone. Also the strains showing the same MIC pattern and farm source, but different O serotype, revealed to be different clonal origin. The strains that showed the same or similar MIC pattern, farm source, and O serotype, revealed identical or similar PFGE pattern, which seemed to belong to be one clone. Meanwhile, horizontal transfer of R plasmid seems to be common in APEC with regardless of O serotype and clone of the strains. These results indicate that rapid and accurate epidemiological survey of APEC can be possible by the combination of O serotyping, plasmid profiling and PFGE analysis following the classification of them into groups of antimicrobial drug resistance pattern.
Key Words: avian pathogenic Escherichia coli (APEC), molecular epidemiological analysis, O serotype, pulsed-field gel electrophoresis (PFGE), restriction endonuclease analysis (REA)


About
Browse articles
For contributors
Policy
Editorial Office
#401-1, 85 Bldg., College of Veterinary Medicine, Seoul National University
1 Gwanak-ro, Gwanak-gu, Seoul 08826, Korea
Tel: +82-2-880-1229    Fax: +82-2-878-9762    E-mail: jvs@ksvs.or.kr                

Copyright © 2022 by The Korean Society of Veterinary Science.

Developed in M2PI

Close layer
prev next