Korean J Vet Res > Volume 54(1); 2014 > Article
Korean Journal of Veterinary Research 2014;54(1):39-48.
DOI: https://doi.org/10.14405/kjvr.2014.54.1.39    Published online March 25, 2014.
Prevalence of virulence and cytolethal distending toxin (CDT) genes in thermophilic Campylobacter spp. from dogs and humans in Gyeongnam and Busan, Korea
Hyun-Ho Cho1, Sang-Hyun Kim2, Wongi Min3, Bok-Kyung Ku1, Yong-Hwan Kim3
1Animal, Plant and Fisheries Quarantine and Inspection Agency
2Viral infectious Disease Research Center, Korea Research Institute of Bioscience & Biotechnology
3College of Veterinary Medicine, Research Institute of Life Science, Institute of Animal medicine, Gyeongsang National University
The prevalence of thermophilic Campylobacter (C.) spp. in stray, breeding, and household dogs was 25.2, 12.0, and 8.8%, respectively. C. jejuni and C. upsaliensis were the predominant Campylobacter spp. from household dogs. cdtA, cdtB, and cdtC were detected by PCR in all isolates. Despite the high cytolethal distending toxin (CDT) gene prevalence, only 26 (31%) C. jejuni strains and one (15.3%) C. coli strain showed evidence of CDT production in HEp-2 cell cytotoxicity assays. Virulence-associated genes detected in the C. jejuni and C. coli isolates were cadF, dnaJ, flaA, racR, ciaB, iamA, pldA, virB11, ceuE, and docC. cadF, dnaJ, flaA, and ceuE were found in all C. jejuni and C. coli isolates. When detecting Guillain-Barr$acute{e}$ syndrome-associated genes (galE, cgtB, and wlaN), galE was identified in all isolates. However, cgtB and wlaN were more prevalent in C. jejuni isolates from humans than those from dogs. Adherence and invasion abilities of the C. jejuni and C. coli strains were tested in INT-407 cells. A considerable correlation (adjusted $R^2$= 0.678) existed between adherence and invasion activities of the Campylobacter spp. isolates.
Key Words: adherence/invasion ability, Campylobacter spp., dog, Guillain-Barr$acute{e}$ syndrome-associated genes, virulence genes

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