| Characterization of antimicrobial resistance and application of RFLP for epidemiological monitoring of thermophilic Campylobacter spp. isolated from dogs and humans in Korea |
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Hyun-Ho Cho1, Sang-Hyun Kim2, Wongi Min3, Bok-Kyung Ku1, Jong-Hyun Kim4, Yong-Hwan Kim3 |
1Animal, Plant and Fisheries Quarantine and Inspection Agency
2Viral Infectious Disease Research Center, Korea Research Institute of Bioscience & Biotechnology
3College of Veterinary Medicine, Research Institute of Life Science, Institute of animal medicine, Gyeongsang National University
4Division of Enteric Bacteria Infections, Center for Infectious Disease, National Institute of Health |
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| Abstract |
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An antimicrobial susceptibility test was conducted to compare the resistance rates among Campylobacter spp. isolates from dogs (n = 50) raised under diverse conditions and humans (n = 50). More than 60% of Campylobacter (C.) jejuni from dogs and humans showed resistance to nalidixic acid, enrofloxacin and ciprofloxacin. C. jejuni isolates from humans showed higher resistance to tetracycline (83.3%) and ampicillin (91.3%) than those from dogs. None of the C. jejuni or Campylobacter coli isolates from humans or dogs were resistant to erythromycin. Overall, 85% of Campylobacter spp. isolates showed a multidrug resistant phenotype. Nucleotide sequencing analysis of the gryA gene showed that 100% of $NA^R/CIP^R$ C. jejuni isolates from dogs and humans had the Thr-$86^{th}$-Ile mutation, which is associated with fluoroquinolone resistance. flaA PCR restriction fragment length polymorphism (RFLP) typing to differentiate the isolates below the species level revealed 12 different clusters out of 73 strains. The human isolates belonged to eight different RFLP clusters, while five clusters contained dog and human isolates. |
| Key Words:
antibiotic resistance, Campylobacter spp., DNA gyrase, point mutation, RFLP |
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