Korean J Vet Res > Volume 55(2); 2015 > Article
Korean Journal of Veterinary Research 2015;55(2):81-88.
DOI: https://doi.org/10.14405/kjvr.2015.55.2.81    Published online June 24, 2015.
Selection and immunomodulatory evaluation of lactic acid bacteria suitable for use as canine probiotics
Su-Min Park, Ho-Eun Park, Wan-Kyu Lee
College of Veterinary Medicine, Chungbuk National University
개 생균제 사용에 적합한 유산균주의 선발 및 면역활성 평가
박수민, 박호은, 이완규
충북대학교 수의과대학
Abstract
This study was conducted to isolate lactic acid bacteria (LAB) from dog intestine and identify potential probiotic strains for canine use. One hundred and one LAB were isolated from feces of 20 healthy dogs. Acid, bile, and heat resistance along with adherence to Caco-2 cells and antimicrobial activity against pathogens were examined. To analyze immunomodulative effects, the production of nitric oxide (NO), TNF-${alpha}$, and IL-$1{eta}$ was measured using RAW 264.7 macrophages. Additionally, RAW BLUE cells were used to evaluate nuclear factor-${kappa}B$ (NF-${kappa}B$) generation. Ultimately, three strains were selected as canine probiotics and identified as Lactobacillus reuteri L10, Enterococcus faecium S33, and Bifidobacterium longum B3 by 16S rRNA sequence analysis. The L10 and S33 strains showed tolerance to pH 2.5 for 2 h, 1.0% Oxgall for 2 h, and $60^{circ}C$ for 5 min. These strains also had strong antimicrobial activity against Escherichia coli KCTC 1682, Salmonella Enteritidis KCCM 12021, Staphylococcus aureus KCTC 1621, and Listeria monocytogenes KCTC 3569. All three strains exerted better immunomodulatory effects than Lactobacillus rhamnosus GG (LGG), a well-known commercial immunomodulatory strain, based on NO, NF-${kappa}B$, IL-$1{eta}$, and TNF-${alpha}$ production. These results suggested that the three selected strains could serve as canine probiotics.
Key Words: Bifidobacterium longum, canine probiotics, Enterococcus faecium, immunomodulation, Lactobacillus reuteri


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