Angled Capillary Method for Determining Erythrocyte Sedimentation Rate of Goat

Shin, Sung-shik; Lee, Bang-whan; Shin, Jong-uk

Korean Journal of Veterinary Research 1986;26(1):187-194.

Sung-shik Shin¹, Bang-whan Lee¹, Jong-uk Shin²

¹Department of Veterinary Medicine, College of Agriculture, Chonnam National University
²Department of Veterinary Medicine, College of Agriculture, Gyeongsang National University

경사모세관법(傾斜毛細管法)에 의한 산양혈액(山羊血液)의 적혈구침강율(赤血球沈降率) 측정(測定)

신성식¹, 이방환¹, 신종욱²

¹전남대학교 농과대학 수의학과
²경상대학교 농과대학 수의학과

Abstract

The purpose of this study is to develop a new method which enables the goat ESR to be used as an effective clinical test. Blood samples were taken from 61 Korean native goats aged above one year old and the effect of tube inclinations, tube bores, tube lengths, environmental temperatures during tests and packed erythrocyte volumes (PCV) on the ESR were observed. The results were summarized as follows. 1. The ESR/hr using capillary hematocrit tube (Micro-Ht-tube) was gradually increased as the tube angle inclined from 90 (vertical) to 15 degrees and the best angle in view of both security and fast sedimentation rate was found to be an angle of 45 degrees. 2. The 45-degree angled ESR ($45^{circ}$-ESR) increased as the diameter of tube bore decreased. 3. The tube length did not affect the $45^{circ}$-ESR in percent. 4. The $45^{circ}$-ESR increased with the increased environmental temperature during the ESR test. 5. The heparinized Micro-Ht tubes did not affect the $45^{circ}$-ESR of EDTA-blood in healthy group but in anemic group. In the anemic group, the ESRs by the heparinized Micro-Ht-tubes were slightly higher than those by non-heparinized Micro-Ht-tubes. 6. By using the autologous plasma, PCV of the blood was adjusted to be 10, 20, 30, 40 and 50ml/100ml and $45^{circ}$-ESRs were determined in the Micro-Ht-tubes. The $45^{circ}$-ESRs increased as the values of PCV decreased. The regression of the $45^{circ}ESR$ to PCV was curvilinear with the second degree polynomial, $Y=42.1838-1.7355X+0.0180X^2$(r=0.9997). 7. The $45^{circ}$-ESR/hr, using non-heparinized Micro-Ht-tubes at $20^{circ}C$, was determined in 35 healthy Korean native goats. The average PCV was $30.6{pm}1.4ml/100ml$. The observed ESR values averaged $6.8{pm}1.7%$ and the corrected ESR values to the standard PCV of 31ml/100ml averaged $6.5{pm}1.2%$. From these results, the angled capillary tube method was found to be desirable ESR test of goat blood in which EDTA-blood is filled in nonheparinized Micro-Ht-tubes held at an angle of 45 degrees for an hour at $20^{circ}C$.