Korean Journal of Veterinary Research 1989;29(4):541-548.
Immunohistochemistry for detection of Aujeszky's disease virus antigens: Protein A-gold labeling of ultrathin sections for electron microscopy
Soon-bok Kim
College of Veterinary Medicine, Gyeongsang National University
오제스키병 바이러스 항원검출을 위한 면역조직화학적 연구 : 전자현미경적 관찰을 위한 초박절편내 protein A-gold labeling
경상대학교 수의과대학
The present study was carried out to determine viral antigens and its morphogenesis in the ultrathin frozen and araldite sections of cell cultures infected with ADV by protein A-gold labeling. ADV antigens were labeled with 10nm gold probes, and electron-dense gold particles were mainly present on viral nucleocapsids and viral envelopes. Immunogold labeling in the ultracryosections showed a very low degree of interaction with tissue structures. Immunogold labeling in the ultrathin cryosections can be useful tool for the detection of ADV antigens, and the technique also may provide its great potential for immunocytochemical studies on various virus-host cell Interactions.
Key Words: protein A-gold labeling, Aujeszky's disease virus antigen, ultrathin cryosections

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