| Immunohistochemical study on the expression of bcl-2 protein during follicular development and atresia in the rat ovary |
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Phil-ok Koh1, Sung-yoon Jeong2, Gyeong-jae Cho2, Wan-sung Choi2, Soo-dong Kwak1 |
1Institute of Animal Medicine, College of Veterinary Medicine Gyeongsang National University
2Department of Anatomy, College of Medicine Gyeongsang National University |
| 흰쥐 난포의 성장과 퇴화에 따른 bcl-2 단백질 발현에 관한 면역조직화학적 연구 |
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고필옥1, 정성윤2, 조경제2, 최완성2, 곽수동1 |
1경상대학교 수의과대학 동물의학연구소
2경상대학교 의과대학 해부학교실 |
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| Abstract |
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In the mammalian ovary, follicular development and atresia continuously occur during the reproductive cycles. Follicular atresia occurs through granulosa cell apoptosis. Apoptosis is known as the physiological cell death, which is regulated by bcl-2 gene family. In the bcl-2 gene family, bcl-2 and bcl-xLong are known as inhibitors of apoptosis, whereas bax and bcl-xShort are known as inducer of apoptosis. We thought that bcl-2 protein is associated with follicular development and atresia. But it is not known that the distribution of cells containing bcl-2 protein during follicular development and atresia. Therefore, to examine the distribution of cells with bcl-2 protein during ovarian follicular development and atresia, the immunohistochemistry was used in the rat ovary. Bcl-2 immunoreactivity was localized in the interstitial cells, theca externa cells and granulosa cells around of antrum. All positive signals were observed in the cytoplasm of these cells. Positive signals were strongly observed in the interstitial and theca externa cells of growing antral follicles. While, positive signals were weakly observed in these cells from atretic antral follicles. Positive signals were very weakly observed in the granulosa cells of growing and atretic antral follicles. According to these data, we suggested that bcl-2 proteins which were strongly expressed in the interstitial cells and theca externa cells of growing antral follicles inhibit follicular atresia. And we purposed that bcl-2 proteins regulated follicular development and atresia through the action of bcl-2 gene family. |
| Key Words:
apoptosis, bcl-2, immunohistochemistry, ovary, rat |
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