| Specific detection of Salmonella serogroup D1 by polymerase chain reaction(PCR) for sefA gene |
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Moo-hyung Jun1, Tae-joong Kim1, Kyung-soo Chang1, Kyong-im Kang1, Kui-hyun Kim1, Ki-seok Kim2, Sang-sik Yoo3, Hyun-soo Kim1, Kwang-soon Shin1, Chul-joong Kim1 |
1College of Veterinary Medicine, Chungnam National University
2National Veterinary Research and Quarantine Services
3Taejon City Institute of Health and Environment |
| SefA 유전자 PCR에 의한 Salmonella serogroup D1의 특이적 검출 |
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전무형1, 김태중1, 장경수1, 강경임1, 김귀현1, 김기석2, 유상식3, 김현수1, 신광순1, 김철중1 |
1충남대학교 수의과대학
2국립수의과학검역원
3대전보건환경연구원 |
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| Abstract |
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Sal enteritidis thin fimbriae, SEF14, were found to be restricted to the predominantly poultry-associated members of the Salmonella serogroup D1 that are considered as the important pathogens in poultry industry. SefA together with sefB and sefC encode the proteins involved in SEF14 biosynthesis. In order to develop the rapid and specific detection methods for Salmonella serogroup D1, a PCR technique for the amplification of sefA gene was established, and its specificity and sensitivity were investigated with various microorganisms. The bacterial genomic DNA was extracted by colony-picking and rapid boiled-lysate technique. In comparison of Sef I and Sef II primers used in the PCR, Sef I primer for sefA gene of 513bp showed higher specificity than that of Sef II. The established PCR was as sensitive as to detect 1pg of Sal enteritidis DNA. When 73 strains in 28 genera including the reference strains and the field isolates of various Salmonella serotypes, Bacillus subtilis, Bordetella bronchisepdca, E coli, Listeria spp., Micrococcus luteus, Rhodococcus equi, Staphylococcus spp., Streptococcus spp., Vibrio parahemolyticus, Yersinia spp. were studied, the established PCR yielded specifically positive results with only Salmonella serogroup D1. The results suggested that the PCR for sefA gene could be a potential candidate among the specific detection methods for Salmonella serogroup D1. |
| Key Words:
PCR, sefA, Salmonella serogroup Dl, avian salmonellosis |
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