Isolation and characteristics of serotype F staphylococcal phage singly converting staphylokinase

Park, Cheong-kyu; Seo, Mi-sook

Korean Journal of Veterinary Research 2000;40(1):49-55.

Cheong-kyu Park, Mi-sook Seo

College of Veterinary Medicine, Kyungpook National University

Staphylokinase 단독변환 혈청형 F 포도구균 phage의 분리 및 특성

박청규, 서미숙

경북대학교 수의과대학

Abstract

Lysogenic conversion of Staphylococcus aureus to loss of ${eta}-hemolysin$ production by serological group F phages is always associated with gain in staphylokinase production. In this study, the new phages belonging to serotype F were detected during the course of isolation of phages from Staph aureus of bovine origin and some characteristics of the new phages isolated were investigated. The new phages, ${phi}470$ and ${phi}499$, isolated from Staph aureus producing ${eta}-hemolysin$ and staphylokinase(${eta}^+;K^+$) were found to convert ${eta}^+;K^+$ strain to ${eta}^+K;^+$, Staph aureus strains lysogenized by this serotype F single-converting phage ${phi}470$ or ${phi}499$ could be again lysogenized with serotype F double-converting phage ${phi}506$. The frequency of lysogenization of indicator strains by serotype F single-converting phage was 100%, whereas the frequency for serotype F double-converting phage ${phi}506$ varied from 4.2% to 97.6% according to the indicator strains. The indicator strain lysogenized with phage ${phi}470$ was resistant to phage ${phi}499$, and vice versa, but not to phage ${phi}506$. Therefore, phage ${phi}470$ and ${phi}499$ were shown to be identical by immunity test.

Key Words: Staphylococcus aureus, staphylokinase, single-lysogenic conversion, lysogenization, bacteriophage