Korean Journal of Veterinary Research 2005;45(2):169-177.
Development of the immunochromatographic strip for the rapid detection of Listeria spp.
Byeong-yeal Jung1, Suk-chan Jung2, Jong-man Kim3
1Veterinary Pharmacology Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry
2Livestock Products Standard Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry
3Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry
Listeria 속균 신속 검출을 위한 면역크로마토그라피 킷트 개발
정병열1, 정석찬2, 김종만3
1농림부 국립수의과학검역원 동물약품과
2농림부 국립수의과학검역원 축산물규격과
3농림부 국립수의과학검역원 세균과
Abstract
We developed the one-step strip based on an immunochromatographic (IC) assay for the rapid detection of Listeria spp. Genus-specific monoclonal antibody to flagella of L. monocytogenes was conjugated with 40 nm colloidal gold particles which were prepared in our laboratory. The specificity of the IC strip was tested with pure cultured bacteria. All strains of the genus of Listeria spp. yielded positive reactions and 12 strains of non-Listeria were negative, resulting in a specificity of 100%. L. monocytogenes was artificially inoculated in raw pork macerated with listeria enrichment broth. And then it was 10-fold diluted from $8.7{ imes}10^6$ to 8.7 CFU/ml. L. monocytogenes could be detected at a minimum of $8.7{ imes}10^5CFU/ml$ before enrichment, $8.7{ imes}10^2CFU/ml$ after primary enrichment and 8.7 CFU/ml after secondary enrichment, respectively. These results indicated that the IC strip exhibited high specificity and sensitivity in the detection of Listeria spp.
Key Words: Listeria, immunochromatographic strip, colloidal gold


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