| Development of high-performance liquid chromatography methods for the anticoccidials: toltrazuril and diclazuril |
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Kyung Hun Jeong, Miyoung Jeong, Hae-Chul Park, Md Akil Hossain, Daegyun Kim, Kwang-Jick Lee, Jeong Woo Kang |
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Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency |
| 고속액체크로마토그래피를 이용한 항원충제 분석법 개발: 톨트라주릴 및 디클라주릴 |
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정경훈, 정미영, 박해철, 아킬후세인, 김대균, 이광직, 강정우 |
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농림축산검역본부 동물약품평가과 |
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| Abstract |
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This study was undertaken to develop new analytical methods for assessment of anticoccidials. High-performance liquid chromatography (HPLC) was found to be a fast, reliable, and practical method. The anticoccidials used in this experiment were toltrazuril and diclazuril, and the analysis factors were specificity, linearity, accuracy, repeatability, and intermediate precision. The linearity of each anticoccidial was better than 0.99, and the accuracies were 99.5% and 99.1% with relative SD of 0.5 and 0.4, respectively. To assess whether the developed HPLC method could be effectively applied, toltrazuril and diclazuril post-market veterinary products (five products) that are currently sold were tested. The results revealed no non-compliant items and the method was applied successfully. Therefore, the newly developed HPLC method for anticoccidial assessment described in this study may be useful as a reference method in the Korean Standards of Veterinary Pharmaceuticals for the analysis of toltrazuril and diclazuril. |
| Key Words:
coccidiostats, diclazuril, high performance liquid chromatography, toltrazuril |
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