Korean Journal of Veterinary Research 1999;39(4):760-764.
Prevalence of tissue antigen and serum antibody for porcine reproductive and respiratory syndrome in Cheju
Seong-hyun Ho1, Yong-chul Jun1, Choi-kyu Park2, Chang-hee Lee2, Jong-hee Bae1
1Veterinary Diagnostic Laboratory, Department of Veteinary Medicine, College of Agriculture, Cheju National University
2National Veterinary Research and Quarantine Service
Abstract
A totoal of 219 pigs, 109 necropsy-pigs at the diagnostic laboratory of Cheju National University and 110 slaughter-pigs in Cheju, were evaluated for the prevalence of tissue antigen and serum antibody for spontaneus porcine reproductive and respiratory syndrome(PRRS). Tissues from 219 pigs examined for PRRS viral antigen by immmunohistochemistry included lung(cranio-ventral lobes and dorso-caudal lobes), tonsil, tracheobronchial lymph node, mesenteric lymph node, heart, kidney, liver, spleen, testis, ovary, brain, and spinal cord. Sera from 180 pigs were tested for the presence of antibody to PRRS virus by the indirect fluorescent antibody assay (IFA). In the examination of serum antibody and tissue antigen for PRRS virus, serum antibody titers were considered as positive in 10%(18/180) of animals tested and PRRS viral antigen was detected in tissues of 4%(9/219) of the pigs. PRRS virus tissue antigen was most commonly detected by immunohistochemistry in the cranio-ventral lobe and tonsil. We also confirmed the distribution of tissue antigen and prevalence of serum antibody to PRRS virus in Cheju. The detection of viral antigen by immunohistochemistry in tonsils and cranio-ventral lobes proved to be a very useful method for PRRS diagnosis.
Key Words: PRRS, antigen prevalence, seroprevalence, immunohistochemistry


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