| Production of monoclonal antibody to 45 kDa somatic protein of Trichuris suis |
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Jong-Kyung Lee1, Jung-Tae Kim2, Hun-Su Seo3, Jong-Yeol Park4, Hee-Jeong Yun5 |
1J.E.S
2Evergreen Animal Hospital
3Pfizer Animal Health Korea
4Bayer Korea
5College of Veterinary Medicine, Seoul National University |
| 돼지편층의 45kDa 항원단백질에 대한 단클론항체 생산 |
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이종경1, 김종태2, 서흔수3, 박종열4, 윤희정5 |
1제스
2늘푸른동물병원
3한국화이자동물약품(주)
4바이엘코리아(주)
5서울대학교 수의과대학 |
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| Abstract |
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Trichnuris suis does not excrete eggs during larval stage as well as in particular adult stage, It is impossible to diagnose by use of fecal examination method in those periods. Therefore, serological diagnostic method can be very useful for those stages. In order to produce monoclonal antibody, specific somatic and secretory-excretory (SE) antigens of T. suis were identified and analyzed by SDS-PAGE and Western blot. Monoclonal antibody-producing hybridoma cells were cloned, which were made of popliteal lymph node of BALB/c mice immunized with a 45 kDa somatic antigen of T. suis. Five clones (1B9, 2C4, n2C5, 2D7 and 2D8) showing strong responses to T. suis antigens were selected and the isotype identified. All monoclonal antibodies were IgG1 isotype and the light chains were k chain. Established monoclonal antibodies reacted specifically to somatic and SE antigens of T. suis and did not cross-reacted to antigens of ascaris suum, trichuris vulpis, or Trichinella spiralis. The sensitivity of somatic and SE antigens against these monoclonal antibodies were significant (p<0.01) associated with those of positive and negative sera. |
| Key Words:
Trichuris suis, somatic, secretory-excretory antigens, monoclonal antibody, western blot, antigen-capture sandwich ELISA |
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