Isolation and identification of Moraxella cuniculi from a rabbit with keratoconjunctivitis

Yang, Dong-Kun; Kim, Ha-Hyun; Yoo, Jae-Young; Lim, Suk-Kyung; Yoon, Soon-Seek; Cho, In-Soo

doi:2017.57.3.201

Korean J Vet Res > Volume 57(3); 2017 > Article

Brief Communication

Korean Journal of Veterinary Research 2017;57(3):201-204.
DOI: https://doi.org/10.14405/kjvr.2017.57.3.201 Published online November 27, 2017.

Isolation and identification of Moraxella cuniculi from a rabbit with keratoconjunctivitis

Dong-Kun Yang, Ha-Hyun Kim, Jae-Young Yoo, Suk-Kyung Lim, Soon-Seek Yoon, In-Soo Cho

Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs

Abstract

A Gram-negative, catalase- and oxidase-positive, coccus-shaped bacterium was isolated from a rabbit with keratoconjunctivitis. Colonies of the isolate were round, smooth, and exhibited hemolytic activity on 5% sheep blood agar. Scanning electron microscopy revealed 0.4 to $0.5{mu}m$ diameter oval cocci. Partial 16S rRNA gene (1446 bp) sequence analysis demonstrated the isolate had significant homology with the Moraxella cuniculi CCUG2154 strain isolated from a rabbit in Germany in 1973. Our isolate was designated as APQAB1701. Antibiotic susceptibility tests demonstrated that APQAB1701 was sensitive to 24 antibiotics; 3 of the antibiotics (nalidixic acid, spectinomycin, and colistin) had minimal inhibitory concentrations ${geq}32{mu}g/mL$ against the isolate.

Key Words: Moraxella cuniculi, identification, isolation, rabbits